Analysis of ACE2 in polarized epithelial cells : surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus
Identifieur interne : 004484 ( Main/Exploration ); précédent : 004483; suivant : 004485Analysis of ACE2 in polarized epithelial cells : surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus
Auteurs : XIAOFENG REN [Allemagne] ; Jörg Glende [Allemagne] ; Marwan Al-Falah [Allemagne] ; Victor De Vries [Allemagne] ; Christel Schwegmann-Wessels [Allemagne] ; XIUXIA QU [République populaire de Chine] ; LEI TAN [République populaire de Chine] ; Thomas Tschernig [Allemagne] ; HONGKUI DENG [République populaire de Chine] ; Hassan Y. Naim [Allemagne] ; Georg Herrler [Allemagne]Source :
- Journal of general virology [ 0022-1317 ] ; 2006.
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- Pascal (Inist)
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Abstract
The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.
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uniqKey="Xiaofeng Ren" last="Xiaofeng Ren">XIAOFENG REN</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Institut fur Virologie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Glende, Jorg" sort="Glende, Jorg" uniqKey="Glende J" first="Jörg" last="Glende">Jörg Glende</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Institut fur Virologie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Al Falah, Marwan" sort="Al Falah, Marwan" uniqKey="Al Falah M" first="Marwan" last="Al-Falah">Marwan Al-Falah</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Institut fur Physiologische Chemie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="De Vries, Victor" sort="De Vries, Victor" uniqKey="De Vries V" first="Victor" last="De Vries">Victor De Vries</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Institut für funktionelle und angewandte Anatomie, Medizinische Hochschule Hannover, Carl-Neuberg-StraBe</s1><s2>30625 Hannover</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Schwegmann Wessels, Christel" sort="Schwegmann Wessels, Christel" uniqKey="Schwegmann Wessels C" first="Christel" last="Schwegmann-Wessels">Christel Schwegmann-Wessels</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Institut fur Virologie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Xiuxia Qu" sort="Xiuxia Qu" uniqKey="Xiuxia Qu" last="Xiuxia Qu">XIUXIA QU</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Lei Tan" sort="Lei Tan" uniqKey="Lei Tan" last="Lei Tan">LEI TAN</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Tschernig, Thomas" sort="Tschernig, Thomas" uniqKey="Tschernig T" first="Thomas" last="Tschernig">Thomas Tschernig</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Institut für funktionelle und angewandte Anatomie, Medizinische Hochschule Hannover, Carl-Neuberg-StraBe</s1><s2>30625 Hannover</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Naim, Hassan Y" sort="Naim, Hassan Y" uniqKey="Naim H" first="Hassan Y." last="Naim">Hassan Y. Naim</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Institut fur Physiologische Chemie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Herrler, Georg" sort="Herrler, Georg" uniqKey="Herrler G" first="Georg" last="Herrler">Georg Herrler</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Institut fur Virologie, Tierarztliche Hochschule Hannover, Bunteweg 17</s1><s2>30559 Hannover</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell surface</term><term>Coronavirus</term><term>Epithelial cell</term><term>Microbiology</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Cellule épithéliale</term><term>Surface cellulaire</term><term>Microbiologie</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>République populaire de Chine</li></country><region><li>Basse-Saxe</li><li>Pékin</li></region><settlement><li>Hanovre</li><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Xiaofeng Ren" sort="Xiaofeng Ren" uniqKey="Xiaofeng Ren" last="Xiaofeng Ren">XIAOFENG REN</name></region><name sortKey="Al Falah, Marwan" sort="Al Falah, Marwan" uniqKey="Al Falah M" first="Marwan" last="Al-Falah">Marwan Al-Falah</name><name sortKey="De Vries, Victor" sort="De Vries, Victor" uniqKey="De Vries V" first="Victor" last="De Vries">Victor De Vries</name><name sortKey="Glende, Jorg" sort="Glende, Jorg" uniqKey="Glende J" first="Jörg" last="Glende">Jörg Glende</name><name sortKey="Herrler, Georg" sort="Herrler, Georg" uniqKey="Herrler G" first="Georg" last="Herrler">Georg Herrler</name><name sortKey="Naim, Hassan Y" sort="Naim, Hassan Y" uniqKey="Naim H" first="Hassan Y." last="Naim">Hassan Y. Naim</name><name sortKey="Schwegmann Wessels, Christel" sort="Schwegmann Wessels, Christel" uniqKey="Schwegmann Wessels C" first="Christel" last="Schwegmann-Wessels">Christel Schwegmann-Wessels</name><name sortKey="Tschernig, Thomas" sort="Tschernig, Thomas" uniqKey="Tschernig T" first="Thomas" last="Tschernig">Thomas Tschernig</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Xiuxia Qu" sort="Xiuxia Qu" uniqKey="Xiuxia Qu" last="Xiuxia Qu">XIUXIA QU</name></noRegion><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><name sortKey="Lei Tan" sort="Lei Tan" uniqKey="Lei Tan" last="Lei Tan">LEI TAN</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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